Journal: Journal of immunology (Baltimore, Md. : 1950)
Article Title: Impairment of dendritic cell functionality and steady-state number in obese mice.
doi: 10.4049/jimmunol.177.9.5997
Figure Lengend Snippet: FIGURE 1. Immune responsiveness to KLH of ob/ob mice. A, Spleen cells from KLH-immunized control (CT, ) and ob/ob (f) mice were cultured with 0–50 g/ml KLH (five mice per group). Each stimulatory condition was done in triplicate. Results are expressed as mean of [methyl-3H]thymidine incorporation SEM. †, p 0.05 by a two-way ANOVA. B, Spleen cells from KLH-immunized control () and ob/ob (f) mice were cultured with 0 or 1 g/ml Con A (five mice per group). Each stimulatory condition was done in triplicate. Results are expressed as mean of [methyl-3H]thymidine incorporation SEM. C, Determination by ELISA of IL-10 and IFN- concentrations in culture supernatants of KLH-immunized control () and ob/ob (f) mice. All tests were done in duplicate. D, Determination by ELISA of total IgG, IgG1, and IgG2a anti-KLH Ab levels in individual sera of nonimmunized and KLH-immunized control () and ob/ob (f) mice. All tests were done in duplicate. Student’s t test: , p 0.05. E, Determination by FACS of percentages of DCs in the spleen of nonimmunized control (left panel) and ob/ob mice (right panel). Cells were double-stained using anti- FITC-coupled MHCII and PE-coupled anti-CD11c Abs. The experiment was performed on five animals, and the difference between both groups was significant using the Student t test; , p 0.05.
Article Snippet: Leptin concentration was determined using the ELISA kit DuoSet mouse leptin (R&D Systems).
Techniques: Control, Cell Culture, Enzyme-linked Immunosorbent Assay, Staining
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![FIGURE 1. Immune responsiveness to KLH of ob/ob mice. A, Spleen cells from KLH-immunized control (CT, ) and ob/ob (f) mice were cultured with 0–50 g/ml KLH (five mice per group). Each stimulatory condition was done in triplicate. Results are expressed as mean of [methyl-3H]thymidine incorporation SEM. †, p 0.05 by a two-way ANOVA. B, Spleen cells from KLH-immunized control () and ob/ob (f) mice were cultured with 0 or 1 g/ml Con A (five mice per group). Each stimulatory condition was done in triplicate. Results are expressed as mean of [methyl-3H]thymidine incorporation SEM. C, Determination by <t>ELISA</t> of IL-10 and IFN- concentrations in culture supernatants of KLH-immunized control () and ob/ob (f) mice. All tests were done in duplicate. D, Determination by ELISA of total IgG, IgG1, and IgG2a anti-KLH Ab levels in individual sera of nonimmunized and KLH-immunized control () and ob/ob (f) mice. All tests were done in duplicate. Student’s t test: , p 0.05. E, Determination by FACS of percentages of DCs in the spleen of nonimmunized control (left panel) and ob/ob mice (right panel). Cells were double-stained using anti- FITC-coupled MHCII and PE-coupled anti-CD11c Abs. The experiment was performed on five animals, and the difference between both groups was significant using the Student t test; , p 0.05.](https://pub-med-unpaywalled-images-cdn.bioz.com/pub_med_ids_ending_with_6524/pm17056524/pm17056524__page4_image1.jpg)